License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing generates high-quality cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. This kit supports up to 960 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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Reproducibility is high for low-input samples
Reproducibility is high for low-input samples. FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4), the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A) or the SMART-Seq2 method (Pearson R = 0.496; Panel C). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though SMART-Seq mRNA did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
Back
Gene body coverage is good for all three library preparation methods
Gene body coverage is good for all three library preparation methods. Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer Ultra Low v3 kit produced a slight 3' bias, and the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4) produced a slight 5' bias; however, the overall coverage was fairly even.
Back
Higher sensitivity and better mappability with SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4).
Higher sensitivity and better mappability with the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4 kit). Replicate libraries were generated from 10 pg Mouse Brain Total RNA using SMART-Seq mRNA, the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq mRNA libraries.
Back
Sequencing metrics are consistent across RNA input amounts
Sequencing metrics are consistent across RNA input amounts. 10 pg-10 ng of Human Brain Total RNA were used to generate cDNA libraries in duplicate with the SMART-Seq mRNA kit (an equivalent replacement for SMART-Seq v4). cDNA libraries were amplified using 17, 14, 10, or 7 PCR cycles for the 10 pg, 100 pg, 1 ng, or 10 ng libraries, respectively.
Back
634894: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing generates high-quality cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. This kit supports up to 480 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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Reproducibility is high for low-input samples
Reproducibility is high for low-input samples. FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4), the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A) or the SMART-Seq2 method (Pearson R = 0.496; Panel C). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though SMART-Seq mRNA did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
Back
Gene body coverage is good for all three library preparation methods
Gene body coverage is good for all three library preparation methods. Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer Ultra Low v3 kit produced a slight 3' bias, and the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4) produced a slight 5' bias; however, the overall coverage was fairly even.
Back
Higher sensitivity and better mappability with SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4).
Higher sensitivity and better mappability with the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4 kit). Replicate libraries were generated from 10 pg Mouse Brain Total RNA using SMART-Seq mRNA, the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq mRNA libraries.
Back
Sequencing metrics are consistent across RNA input amounts
Sequencing metrics are consistent across RNA input amounts. 10 pg-10 ng of Human Brain Total RNA were used to generate cDNA libraries in duplicate with the SMART-Seq mRNA kit (an equivalent replacement for SMART-Seq v4). cDNA libraries were amplified using 17, 14, 10, or 7 PCR cycles for the 10 pg, 100 pg, 1 ng, or 10 ng libraries, respectively.
Back
634893: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing generates high-quality cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. This kit supports up to 192 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
Back
Reproducibility is high for low-input samples
Reproducibility is high for low-input samples. FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4), the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A) or the SMART-Seq2 method (Pearson R = 0.496; Panel C). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though SMART-Seq mRNA did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
Back
Gene body coverage is good for all three library preparation methods
Gene body coverage is good for all three library preparation methods. Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer Ultra Low v3 kit produced a slight 3' bias, and the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4) produced a slight 5' bias; however, the overall coverage was fairly even.
Back
Higher sensitivity and better mappability with SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4).
Higher sensitivity and better mappability with the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4 kit). Replicate libraries were generated from 10 pg Mouse Brain Total RNA using SMART-Seq mRNA, the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq mRNA libraries.
Back
Sequencing metrics are consistent across RNA input amounts
Sequencing metrics are consistent across RNA input amounts. 10 pg-10 ng of Human Brain Total RNA were used to generate cDNA libraries in duplicate with the SMART-Seq mRNA kit (an equivalent replacement for SMART-Seq v4). cDNA libraries were amplified using 17, 14, 10, or 7 PCR cycles for the 10 pg, 100 pg, 1 ng, or 10 ng libraries, respectively.
Back
634892: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing generates high-quality cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. This kit supports up to 96 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
Back
Reproducibility is high for low-input samples
Reproducibility is high for low-input samples. FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4), the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A) or the SMART-Seq2 method (Pearson R = 0.496; Panel C). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though SMART-Seq mRNA did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
Back
Gene body coverage is good for all three library preparation methods
Gene body coverage is good for all three library preparation methods. Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer Ultra Low v3 kit produced a slight 3' bias, and the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4) produced a slight 5' bias; however, the overall coverage was fairly even.
Back
Higher sensitivity and better mappability with SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4).
Higher sensitivity and better mappability with the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4 kit). Replicate libraries were generated from 10 pg Mouse Brain Total RNA using SMART-Seq mRNA, the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq mRNA libraries.
Back
Sequencing metrics are consistent across RNA input amounts
Sequencing metrics are consistent across RNA input amounts. 10 pg-10 ng of Human Brain Total RNA were used to generate cDNA libraries in duplicate with the SMART-Seq mRNA kit (an equivalent replacement for SMART-Seq v4). cDNA libraries were amplified using 17, 14, 10, or 7 PCR cycles for the 10 pg, 100 pg, 1 ng, or 10 ng libraries, respectively.
Back
634891: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing generates high-quality cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. This kit supports up to 48 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
Back
Reproducibility is high for low-input samples
Reproducibility is high for low-input samples. FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4), the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A) or the SMART-Seq2 method (Pearson R = 0.496; Panel C). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though SMART-Seq mRNA did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
Back
Gene body coverage is good for all three library preparation methods
Gene body coverage is good for all three library preparation methods. Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer Ultra Low v3 kit produced a slight 3' bias, and the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4) produced a slight 5' bias; however, the overall coverage was fairly even.
Back
Higher sensitivity and better mappability with SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4).
Higher sensitivity and better mappability with the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4 kit). Replicate libraries were generated from 10 pg Mouse Brain Total RNA using SMART-Seq mRNA, the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq mRNA libraries.
Back
Sequencing metrics are consistent across RNA input amounts
Sequencing metrics are consistent across RNA input amounts. 10 pg-10 ng of Human Brain Total RNA were used to generate cDNA libraries in duplicate with the SMART-Seq mRNA kit (an equivalent replacement for SMART-Seq v4). cDNA libraries were amplified using 17, 14, 10, or 7 PCR cycles for the 10 pg, 100 pg, 1 ng, or 10 ng libraries, respectively.
Back
634890: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing generates high-quality cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. This kit supports up to 24 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
Back
Reproducibility is high for low-input samples
Reproducibility is high for low-input samples. FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4), the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A) or the SMART-Seq2 method (Pearson R = 0.496; Panel C). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though SMART-Seq mRNA did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
Back
Gene body coverage is good for all three library preparation methods
Gene body coverage is good for all three library preparation methods. Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer Ultra Low v3 kit produced a slight 3' bias, and the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4) produced a slight 5' bias; however, the overall coverage was fairly even.
Back
Higher sensitivity and better mappability with SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4).
Higher sensitivity and better mappability with the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4 kit). Replicate libraries were generated from 10 pg Mouse Brain Total RNA using SMART-Seq mRNA, the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq mRNA libraries.
Back
Sequencing metrics are consistent across RNA input amounts
Sequencing metrics are consistent across RNA input amounts. 10 pg-10 ng of Human Brain Total RNA were used to generate cDNA libraries in duplicate with the SMART-Seq mRNA kit (an equivalent replacement for SMART-Seq v4). cDNA libraries were amplified using 17, 14, 10, or 7 PCR cycles for the 10 pg, 100 pg, 1 ng, or 10 ng libraries, respectively.
Back
634889: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
License Statement
| ID Number | |
|---|---|
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing generates high-quality cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. This kit supports up to 12 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components You May Also Like Image Data
Back
Reproducibility is high for low-input samples
Reproducibility is high for low-input samples. FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4), the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A) or the SMART-Seq2 method (Pearson R = 0.496; Panel C). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though SMART-Seq mRNA did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
Back
Gene body coverage is good for all three library preparation methods
Gene body coverage is good for all three library preparation methods. Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer Ultra Low v3 kit produced a slight 3' bias, and the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4) produced a slight 5' bias; however, the overall coverage was fairly even.
Back
Higher sensitivity and better mappability with SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4).
Higher sensitivity and better mappability with the SMART-Seq mRNA (an equivalent replacement for SMART-Seq v4 kit). Replicate libraries were generated from 10 pg Mouse Brain Total RNA using SMART-Seq mRNA, the SMARTer Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq mRNA libraries.
Back
Sequencing metrics are consistent across RNA input amounts
Sequencing metrics are consistent across RNA input amounts. 10 pg-10 ng of Human Brain Total RNA were used to generate cDNA libraries in duplicate with the SMART-Seq mRNA kit (an equivalent replacement for SMART-Seq v4). cDNA libraries were amplified using 17, 14, 10, or 7 PCR cycles for the 10 pg, 100 pg, 1 ng, or 10 ng libraries, respectively.
Back
634888: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
License Statement
| ID Number | |
|---|---|
| 385 | This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com. |
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 PLUS Kit is a complete kit designed to first generate high-quality cDNA from ultra-low amounts of total RNA (10 pg–10 ng) and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit (Cat. # R400744 or R400745). This kit supports up to 48 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components Image Data
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R400752: SMART-Seq v4 PLUS Kit
License Statement
| ID Number | |
|---|---|
| 385 | This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com. |
| 275 | SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com. |
The SMART-Seq v4 PLUS Kit is a complete kit designed to first generate high-quality cDNA from ultra-low amounts of total RNA (10 pg–10 ng) and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit (Cat. # R400744 or R400745). This kit supports up to 96 reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Documents Components Image Data
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R400753: SMART-Seq v4 PLUS Kit
